Hyperthermia treatment of cancer cells by the application of targeted silk/iron oxide composite spheres

Magnetic iron oxide nanoparticles (IONPs) are one of the most extensively studied materials for theranostic applications. IONPs can be used magnetic resonance imaging (MRI), delivery therapeutics, and hyperthermia treatment. Silk is a biocompatible material biomedical Previously, we produced spheres made H2.1MS1 bioengineered silk that specifically carried drug to Her2-overexpressing cancer cells. To confer biocompatibility targeting properties IONPs, blended these particles with spider silks. Three silks (MS1Fe1, MS1Fe2, MS1Fe1Fe2) functionalized adhesion peptides F1 F2, were constructed investigated form composite carrying positive or negative charge. Due its highest IONP content, MS1Fe1 was produce from H2.1MS1:MS1Fe blend obtain carrier cell-targeting properties. Composite H2.1MS1:MS1Fe1/IONP at different ratios obtained. Although increased content in resulted an affinity it decreased binding The H2.1MS1:MS1Fe1 prepared ratio 8:2 loaded exhibited ability bind targeted cells similar control without IONPs. Moreover, when exposed alternating field, generated 2.5 times higher heat. They caused almost three percentage apoptosis than particles. blending enabled generation cancer-targeting high nanoparticles, which anti-cancer therapy.